MGC803-CAS9 cell line

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MGC803-CAS9 (Hela-contaminated cells, not for sales) Order

Catalog#

YC-C081-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

MGC803-CAS9

Morphology

Epithelial-like,adherent

Passage ratio

1：3-1：4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MGC803 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MGC803-CAS9 cell line that can maintain long-term, stable and high expression.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MGC803				STR Info (Cell bank) Cell Line:MGC-803
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
D5S818	10	11	12.0		10	11	12.0
D13S317	7	13.3			7	13.3
D7S820	11	12			11	12
D16S539	9	11			9	11
VWA	16	17	18.0		16	17	18.0
TH01	7	9			7	9
AMEL	X	X			X	X
TPOX	12	12			12	12
CSF1PO	9	10	12.0		9	10	12.0

* Hela-contaminated cells, not for sales

Reviewed by ICLAC (ref: 191110). MGC-803 was reported to originally come from gastric carcinoma but several papers from cell repositories in China have shown that it has a high similarity to HeLa. Looking at the STR profiles that we have available, they appear to fall into two groups. One is consistent with HeLa, while the other has additional alleles at many loci e.g. Penta E has "7,10,16,17". This second group of samples may represent a mixture of HeLa and a second cell line; it may also represent a hybrid cell line (e.g., following exposure to a fusogenic agent).

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

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MGC803-CAS9 cell line

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